axiovert 200 m wide-field fluorescence microscope  (Carl Zeiss)

Journal: PLoS ONE

Article Title: Functional Dissection of SseF, a Membrane-Integral Effector Protein of Intracellular Salmonella enterica

doi: 10.1371/journal.pone.0035004

Figure Lengend Snippet: HeLa cells were transfected with a plasmid for the expression of LAMP1-GFP and infected at an MOI of 50 with Salmonella WT [mCherry], sseF [mCherry], or co-infected with sseF [mCherry] and sseG [GFP]. Live cell imaging was performed 5 h p.i. for a period of 5 min. using a Zeiss Axiovert 200 M wide field microscope equipped with an environmental chamber. The rectangles in overview images indicate detail sections shown as time series. The acquisition time is indicated in min∶sec. Green fluorescence (LAMP1-GFP and sseG [GFP]) is shown in white and arrows indicate the extension and contraction of SIF. Note the appearance of extended continuous SIF in WT and sseF / sseG co-infected cells and the fine and short tubular structures that appear in the cell infected with the sseF strain. Scale bar: 10 µm.

Article Snippet: Samples were analyzed using a Zeiss Axiovert 200 M wide-field microscope with an Axiocam MRm camera or a Leica SP5 confocal laser-scanning microscope (CLSM).

Techniques: Transfection, Plasmid Preparation, Expressing, Infection, Live Cell Imaging, Microscopy, Fluorescence